Traditionally, library based approaches have been used to discover VHHs. These methods are well established but can produce antibodies with low affinities, unfavorable developability profiles, and challenges for screening in cell-based contexts. In vivo based discovery methods have the ability to deliver VHHs with:
By monitoring the development of the natural immune response over time, we are able identify high quality VHH leads that are best suited for our partners’ therapeutic applications.
There are many in vivo-based VHH discovery approaches that begin to capture the advantages of the natural immune system. However, these approaches have certain limitations that can affect the success of the discovery effort, including:
In vitro-based discovery approaches have been used for many years to generate VHHs. This process allows for fast capture of VHH hits but has certain limitations that become more pronounced as the applications and functional requirements of these molecules continuously evolve. These limitations include:
Abkyu utilizes a proprietary workflow that leverages next generation sequencing of target-specific B cells over the evolving immune response to identify the best possible leads for our partners’ downstream applications.
Iterative sequencing and empirical testing allow us to build high confidence sequence selection models that enable:
Custom immunization protocols and adjuvant strategies to ensure tailored immune responses
Deep repertoire sequencing and archiving of millions of antigen specific B cells
Machine learning and bioinformatics pipeline for thorough interrogation of VHH repertoire
Transparency and collaboration in everything we do
Regular data and project communications so our partners know exactly what is going on in their projects
Fully owned partner deliverables enable open access to project data
Leverage optimized camelid immunization protocols to achieve precise and effective immune responses
Custom VHH bioinformatics pipelines designed to address intricacies of camelid immune system and VHH molecules
Lead characterization pipelines designed to test VHHs for relevant downstream applications
Boston’s In Vivo VHH Discovery Specialists
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